Figure 1.

UVA light-induced, locally restricted decondensation of chromatin. UVA light (300–400 nm, ~60 J/cm²) leads to chromatin decondensation and expulsion of the chromatin into the extracellular space as indicated by SYTOX green positive staining of decondensed chromatin. This effect is locally restricted to the area of irradiation. Irradiation: 3 min to induce NET formation + intermittent irradiation during live-cell imaging (3 h; frame rate: 15 min/image). Cells were kept in RPMIcomp. + 10 mM HEPES.