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. 2019 Oct 25;10:2428. doi: 10.3389/fimmu.2019.02428

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Copyright © 2019 Neubert, Bach, Busse, Bogeski, Schön, Kruss and Erpenbeck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wavelength-dependency of NET formation correlates with light absorption of riboflavin. UVA and blue light significantly induce NET formation at (A) the same energy-dose and (B) the same photon flux, whereas green light (565 nm) does not induce NETosis under either condition. Statistics: repeated measure one-way-ANOVA with Bonferroni's multiple comparisons test (tested against unstimulated cells). ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. N = 3 independent experiments. Error bars = SEM. Cells kept in RPMIcomp. + 10 mM HEPES. (C) Riboflavin absorbs light in the UVA-blue light region with maxima at 373 and 445 nm. At 375 nm, the absorption is slightly higher (0.138), than at 470 nm (0.130).