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. 2019 Oct 25;13:483. doi: 10.3389/fncel.2019.00483

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Copyright © 2019 Michels, Dolga, Braun, Kisko, Sungur, Witt, Rietschel, Dempfle, Wöhr, Schwarting and Culmsee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Key steps in oxidative phosphorylation and representative oxygen consumption rate measurement with isolated mitochondria from rat brain. (A) The scheme illustrates the electron transport chain with its five different complexes. Modulating compounds used in the Seahorse mitochondrial coupling assay are highlighted in red. (B) An exemplary measurement of the oxygen consumption rate (OCR) from isolated rat brain mitochondria is shown together with key parameters of mitochondrial respiration, which were calculated from the data. Five technical replicates are displayed as mean ± SD. IMS, intermembrane space; MM, mitochondrial membrane; OCR, oxygen consumption rate; Oligo, oligomycin; AA, antimycin A.