Figure 4.

A representative sample to demonstrate the analysis using the Step Response Method, using data from the same experiment shown in Figure 3. (A) The measured pressure P over 7 increasing pressure steps in an untreated mouse eye (black tracing; data repeated from Figure 3C). Purple line shows the applied pressure, P_a. Green highlights indicate the stable pressure for each step. Inset shows pressure data from a single step (black tracing) fit by the analytical model (red shading) given by Equations (8)–(10). (B) Residuals between the model fit and pressure tracing over all steps, with 95% confidence intervals on the residual for each step indicated by the shading. (C) Pressure drop across the capillary and flow sensor plotted vs. the measured flow rate, with a linear fit according to Equation (14) yielding estimated values of the slope (1/C_q, see section Calculation of Ocular Compliance using the Step Response Method for details). (D) Ocular compliance, ϕ, plotted against pressure, P_ϕ (filled circles). Red curve represents the best fit by Equation (3), along with 95% confidence bounds (in red shading). Black line and grey shading represent the estimated reference compliance, ϕ_r, and its 95% confidence interval at a reference pressure, P_r, of 13 mmHg (dashed vertical line). Open circles represent system compliance reproduced from Figure 3D.