FIGURE 2.

Targeted mutation of HaREase using the CRISPR/Cas9 system. (A) Schematic diagram of the sgRNA targeting site. The box represents the HaREase exon and the black line indicates the gene locus. The sgRNA target site is located on the sense strand of the exon. The protospacer adjacent motif (PAM) sequence is in red. (B) Sequences of the sgRNA target site in WT and six ΔHaREase mutants. The sgRNA target site sequence is in green and the PAM sequence is in red. (C) Confirmation of HaREase gene silencing in KO compared with WT. (D) Total numbers of bacteria in the midgut third instar larvae of WT and KO. (E) Hematoxylin and eosin stained images of the midgut of fifth instar larvae of WT and KO. Data are mean SD, n = 3. ^∗∗P < 0.01.