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. 2019 Oct 25;10:2514. doi: 10.3389/fimmu.2019.02514

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Copyright © 2019 Satta, Grazia, Caroli, Frigerio, Di Nicola, Raspagliesi, Mezzanzanica, Zaffaroni, Gianni, Anichini and Figini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tumor growth inhibition assay on a panel of TRAIL-R2-positive or negative cells. ScDb-dependent PBL-mediated growth inhibition was performed on several cancer cell lines using scDb concentrations of 0, 0.05, 0.1, 0.5, and 1 μg/mL and an E:T ratio of 5:1 for 48 h (black line) or 96 h (gray line). Non-activated PBLs were used as effectors. The graph represents the mean ± SD of three experiments. In each graph the expression level of TRAIL-R2 as mean fluorescence intensity (MFI) is reported.