. 2019 Oct 23;9(11):1994–2005. doi: 10.1002/2211-5463.12740

Table 1.

List of primers used for insertion of C‐terminal HA‐tag and for SDM.

PrimerID	Description	Sequence (5′–3′)
LDLR_HA1F	HA‐tag first 5 amino acids	`GAGGATGACGTGGCGTACCCATACGATGTTTGAACATCTGCCTGG`
LDLR_HA1R	HA‐tag first 5 amino acids	`CCAGGCAGATGTTCAAACATCGTATGGGTACGCCACGTCATCCTC`
LDLR_HA2F	HA‐tag last 4 amino acids	`GTACCCATACGATGTTCCAGATTACGCTTGAACATCTGCCTGG`
LDLR_HA2R	HA‐tag last 4 amino acids	`CCAGGCAGATGTTCAAGCGTAATCTGGAACATCGTATGGGTAC`
LDLR_D445E_F	SDM_D445E: C>G	`GAATCTACTGGTCTGAGCTGTCCCAGAGAATG`
LDLR_D445E_R	SDM_D445E: C>G	`CATTCTCTGGGACAGCTCAGACCAGTAGATTC`
LDLR_D482H_F	SDM_D482H: G>C, C>T	`CGACGGGCTGGCTGTGCATTGGATCCACAGCAACA`
LDLR_D482H_R	SDM_D482H: G>C, C>T	`TGTTGCTGTGGATCCAATGCACAGCCAGCCCGTCG`
LDLR_C667F_F	SDM_C667F: G>T	`GAGGAGTGAACTGGTTTGAGAGGACCACCC`
LDLR_C667F_R	SDM_C667F: G>T	`GGGTGGTCCTCTCAAACCAGTTCACTCCTC`