Figure 4.

5‐ALA/SFC increased HO‐1 and Nrf2 expression via activation of MAPK signaling pathway in mProx24 cells. (A) After pretreatment with HO‐1 siRNA, representative bands of different groups showed HO‐1, Nrf2, p38, p‐p38, Erk‐1/2, p‐Erk‐1/2, Bcl‐2, and cleaved‐caspase‐3 proteins in mProx24 cells treated with 5‐ALA/SFC. (B) Quantitative densitometry was performed. Data are the mean ± SD. Statistical significance was measured by Student's t‐test. Correlations were determined by Spearman's ranking (n = 3; *P < 0.05, **P < 0.01).