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. 2019 Oct 25;10:1270. doi: 10.3389/fphar.2019.01270

Figure 6.

Figure 6

Sirtuin 6 (SIRT6) and peroxisome proliferator-activated receptors α (PPARα) involved in OSS_128167-mediated hepatitis B virus (HBV) transcriptional repression. (A–C) HepG2.2.15 and HBV-infected HepG2-sodium taurocholate cotransporting polypeptide (NTCP) cells were transfected with plasmids expressing Flag-SIRT6 1 day after transfection of construct expressing PPARα short hairpin ribonucleic acid (shRNA) or scramble control shRNA. The HBV core deoxyribonucleic acid (DNA) level was detected by real-time polymerase chain reaction (PCR) and southern blotting analysis at 4 days posttransfection of Flag-SIRT6. The 3.5-Kb RNA level was determined by real-time PCR analysis at 3 days posttransfection of Flag-SIRT6. β-Actin was used as the internal control. (D–F) HepG2.2.15 and HBV-infected HepG2-NTCP cells were respectively transfected with plasmids Flag-SIRT6 or Flag-PPARα 1 day before OSS_128167 treatment. The HBV core DNA level was detected by real-time PCR and southern blotting analysis at 4 days posttransfection. The 3.5-Kb RNA level was determined by real-time PCR analysis at 3 days posttransfection. β-Actin was used as the internal control. Data represented the mean ± SD of three independent experiments. *:P < 0.05.