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. 2019 Oct 25;13:468. doi: 10.3389/fncel.2019.00468

Figure 2.

Figure 2

MKK7/ c-Jun N-terminal kinase(JNK)/c-Jun cascade was activated under 5K condition, and inhibition of HDACs transcriptionally suppressed MKK7 expression and lowered 5K-evoked JNK/c-Jun activity. (A–C) CGNs in 25K and 5K media for the indicated time course were subjected to WB or reverse transcription (RT)-PCR to detect the levels of phosphorylated-MKK7 (p-MKK7), total MKK7, p-JNK, p-c-Jun (ser73), c-Jun by WB, or mmk7 mRNA by Quantitative PCR (Q-PCR). GAPDH or actin was detected to verify equal loadings. (B,C) The gray densities of MKK7 were analyzed after normalization to GAPDH in ImageJ software. Mean ± SD, *P < 0.05 vs. 25K. (D–F) CGNs in 25K that contained DMSO or 5K media that contained TSA, SAHA, VPA, or M344 for 12 h were subjected to WB to detect the levels of MKK7, p-JNK, p-c-Jun, or c-Jun by WB or Q-PCR to detect the mkk7 mRNA level. (E,F) The gray densities of MKK7 were analyzed as in (B,C). Mean ± SD, *P < 0.05 vs. 25K, #P < 0.05 vs. TSA, SAHA, VPA, or M344, respectively.