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. 2019 Oct 25;13:468. doi: 10.3389/fncel.2019.00468

Figure 4.

Figure 4

Inhibition of HDAC4 rescues 5K-induced apoptosis. (A,B) CGNs were transfected with siNC, siHDAC4-a, or siHDAC4-b together with pCMV-EGFP. After 48 h, neurons were exposed to 5K media for 12 h and subjected to nucleic staining and apoptotic analysis as in Figure 1. Mean ± SD, *P < 0.05 vs. 25K, #P < 0.05 vs. siHDAC4-a or siHDAC4-b. (C–F) CGNs in 25K or 5K that contained LMK235 at the indicated doses for 12 h were subjected to apoptotic analysis by nuclear staining with Hoechst 33258 or PI. Mean ± SD, *P < 0.05 vs. 25K, #P < 0.05 vs. 5K, $P < 0.05 vs. 0.1 μM LMK235, ∧P < 0.05 vs. 0.5 μM LMK235.