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. 2019 Oct 31;6:250. doi: 10.1038/s41597-019-0216-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Validation of sRNA-Seq results by stem-loop RT-qPCR. The log₂ ratios of expression of six miRNAs in PVY^NTN-infected samples versus mock-inoculated samples of potato cv. (a) Désirée or (b) NahG-Désirée as determined by sRNA-Seq (dark grey) or RT-qPCR (light grey). The expressions of miR827 and miR4376 in NahG-Désirée samples were under the limit of quantification. Asterisks indicate statistically significant changes as determined by Student t-test (n = 3; **p-value < 0.05; *p-value < 0.1). miR390 – stu-miR390-5p; miR398 – stu-miR398a-5p; miR408 – stu-miR408b-5p; miR4376 – stu-miR4376-5p.1; miR6022 – stu-miR6022-3p; miR827 – stu-miR827-5p. See Methods for details of the experimental procedure.