Table 1.
Validation of microarray results by RT-qPCR.
| Desiree | Desiree-NahG | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dpi1 | dpi3 | dpi4 | dpi5 | dpi7 | dpi1 | dpi3 | dpi4 | dpi5 | dpi7 | ||||||||||||
| μarray | qPCR | μarray | qPCR | μarray | qPCR | μarray | qPCR | μarray | qPCR | μarray | qPCR | μarray | qPCR | μarray | qPCR | μarray | qPCR | μarray | qPCR | ||
| CAB | cSTD1O21THB | 1.4 | 0.3 | 0.3 | −0.4 | 1.1 | 1.3 | −0.5 | 0.4 | 0.1 | 2.1 | 1 | −0.8 | 0.1 | 0 | 0 | −0.6 | 0.2 | 1.3 | −0.1 | 0.6 |
| MICRO.331.C90 | 0.1 | 0 | 0.5 | 0.1 | −0.1 | −0.1 | −0.1 | 0 | 0.2 | 0 | |||||||||||
| MICRO.331.C84 | 1.2 | 0.6 | 0.6 | −0.6 | −0.6 | −1.1 | −0.5 | −1.4 | −0.7 | −1 | |||||||||||
| MICRO.331.C81 | 3.5 | 2.9 | 2.7 | 3.3 | 3 | −0.8 | −0.5 | 0.6 | 0.5 | −0.8 | |||||||||||
| MICRO.331.C62 | 0.9 | 0.8 | 0.8 | 0.8 | 0.2 | −0.9 | 0.1 | 0 | 0.2 | −0.3 | |||||||||||
| MICRO.331.C60 | 0.2 | −0.1 | 1.8 | −0.1 | −0.3 | −0.4 | 0.2 | −0.6 | 0.5 | 0 | |||||||||||
| MICRO.331.C89 | 1.1 | −0.4 | 2.3 | −0.9 | 0.2 | −1.4 | 1.2 | 0.3 | 1.6 | 0.7 | |||||||||||
| MICRO.331.C79 | 0.6 | −0.1 | 2.2 | −0.5 | −0.4 | −0.3 | −0.2 | −1.8 | 0.4 | 0.1 | |||||||||||
| MICRO.331.C76 | 0.9 | −0.2 | 1.4 | −0.6 | −0.3 | 0.7 | −0.1 | −0.1 | 0.3 | 0.3 | |||||||||||
| MICRO.331.C27 | 1.7 | 0.3 | 2.5 | −0.1 | 1.3 | −1.7 | 0.8 | 1 | 1.3 | 0.4 | |||||||||||
| MICRO.331.C12 | 0.8 | 0.7 | 0.5 | 0.1 | 0.1 | 0.8 | 0 | 0.4 | 0.4 | −0.4 | |||||||||||
| MICRO.331.C9 | 0.5 | 0.1 | 1.8 | −0.1 | 0.1 | 0.3 | 0.5 | 0.4 | 0.8 | 0.3 | |||||||||||
| INV | STMIM75TV | −0.6 | −0.6 | 0.3 | 0 | 0 | −1 | 0.1 | −1.7 | 0.5 | 1 | 0.4 | 1.1 | −0.5 | 1 | 1.4 | 1.1 | 1.5 | 2.2 | 1.8 | 1.7 |
| GBSS1 | MICRO.920.C5 | 0.2 | 0.4 | −0.3 | 0.4 | 0.3 | 0.9 | −1.4 | −0.8 | −0.3 | 0.3 | −0.5 | −2.1 | 0 | −0.3 | −1.3 | −1.5 | −0.3 | −0.3 | −0.5 | −0.1 |
| MICRO.920.C2 | 0.3 | −0.4 | 0.7 | −1.5 | 0 | −0.4 | 0.1 | −1.4 | −0.2 | −0.4 | |||||||||||
| Glu-I | MICRO.2526.C3 | 0.2 | 1.5 | −0.1 | −0.2 | 0 | 1.8 | −0.2 | −0.6 | 0.1 | 1.8 | 0.3 | 4.5 | 0.2 | 0.6 | 0.3 | 2.8 | 0.1 | 2.2 | 0.4 | 3 |
| Glu-II | MICRO.2286.C42 | −0.6 | −0.3 | −0.7 | −0.6 | 0.1 | 0.7 | −0.6 | −1.7 | −0.2 | −1.2 | 0.4 | −0.2 | −0.7 | 2.4 | −0.8 | −1.4 | 2.1 | 2.5 | 3.7 | 4.3 |
| MICRO.2286.C28 | 0 | −0.2 | 0.5 | 0.1 | 0.7 | −0.7 | 0.5 | 0 | 0.6 | −0.5 | |||||||||||
| MICRO.2286.C15 | −0.3 | −0.7 | 0.2 | −0.9 | −0.1 | 0.7 | −0.8 | −0.9 | 1.8 | 3.9 | |||||||||||
| MICRO.2286.C1 | 0 | 0 | 0 | 0.1 | 0.2 | 0 | 0.2 | 0.4 | 0.3 | −0.1 | |||||||||||
| Glu-III | MICRO.6187.C2 | −0.4 | −0.5 | −0.3 | −0.5 | 0.4 | 0.5 | 0.2 | −1 | 0.4 | −0.4 | −0.3 | −0.2 | −1.1 | −0.5 | 1.4 | 0.5 | 3.6 | 3.1 | 3.9 | 4.6 |
| MICRO.6187.C1 | −0.4 | −0.2 | 0.9 | −0.1 | 0.4 | 0.1 | −1.8 | 0.6 | 3.8 | 4.5 | |||||||||||
| PR1b | MICRO.5426.C4 | −0.2 | −0.6 | −1.7 | −0.8 | 0.5 | 1.3 | −1.3 | −1.6 | 1.3 | 1.5 | −1.2 | −3.8 | −2.3 | −2 | −0.4 | 0 | 4.4 | 5.6 | 5.9 | 5.4 |
| RA | MICRO.4141.C1 | 0.2 | −0.6 | −0.4 | −1 | −0.4 | −0.6 | −1.3 | −1.5 | −1.7 | −1.8 | 0.6 | −0.9 | −0.3 | −0.4 | −1 | −0.9 | −0.4 | 0.5 | −0.1 | 0.8 |
Microarray results were validated by analyzing eight biologically relevant genes coding for proteins involved in photosynthesis (chlorophyll a-b binding protein: CAB and RuBisCO activase: RA), defense response (β-1,3-glucanase of three classes: Glu-I, Glu-II, Glu-III and pathogenesis-related protein1b: PR-1b), and sugar metabolism (granule bound starch synthase I: GBSSI and INV: cell wall invertase) by quantitative real-time PCR. Expression values were log2 transformed, and a fold-change difference (log2FC) was calculated for PVYNTN versus mock in cv. Désirée and NahG-Désirée at 1, 3, 4, 5 and 7 dpi are shown in the table. Statistically significant values (p < 0.05) are marked with bold.