Table 2.

Cell plasma membrane integrity after testicular tissue vitrification and warming, determined by trypan blue dye exclusion assay. Controls, testicular tissue analyzed immediately after dissection. Control 2 h and 24 h, testicular tissue analyzed after 2 and 24 h of in vitro culture. Vitrified 0 h, testicular tissue subjected to vitrification and analyzed immediately after warming. Vitrified 2 h and 24 h, testicular tissue subjected to vitrification and analyzed after 2 or 24 h post-warming in vitro culture. Different superscript letters indicate a significant difference (ANOVA; P < 0.05)

Treatment	Plasma membrane integrity (mean ± SE)
Control 0 h	89.67% ± 1.45 a
Control 2 h	86.67% ± 1.33 a
Control 24 h	81.00% ± 4.58 a
Vitrified 0 h	66.00% ± 4.73 b
Vitrified 2 h	59.67% ± 4.18 b
Vitrified 24 h	31.00% ± 3.46 c