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. 2019 Oct 31;9:15759. doi: 10.1038/s41598-019-52294-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterisation of Oct4+ hDFs. (a) Morphology of control GFP+ hDFs and Oct4+ hDFs 6 days post transduction, as determined by light microscopy. Scale bar = 100 μm. (b) Analysis of POU5F1 expression in Oct4+ hFDs relative to GFP+ hDFs 6 days post transduction, as determined by RT-qPCR. Error bars represent ± SD. (c) Western blot analysis of mesenchymal/epithelial markers and Oct4 expression in control GFP+ hDFs and Oct4+ hDFs 6 days post transduction. α-tubulin and β-actin were used as a loading control. Uncropped western blot images are shown in Supplementary Fig. 2a. (d) Analysis of SNAI2, COL1A, CDH1, EPCAM, CRB3 expression in Oct4+ hFDs relative to GFP+ hDFs 6 days post transduction, as determined by RT-qPCR. Error bars represent ± SD. (e) Analysis of cell migration of Oct4+ and GFP+ hDFs, as determined by scratch-wound healing assay. The graph shows cell-free area during time upon making a straight scratch on tissue culture plate. Error bars show ± SE, n = 5.