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. 2019 Oct 31;9:15759. doi: 10.1038/s41598-019-52294-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of NGS using RT-qPCR. Expression levels of selected miRNAs that are differentially expressed based on NGS data, as determined by RT-qPCR. (a) miRNAs up-regulated in Oct4+ hDFs: mir-183, mir-302 cluster members, and miR-9-5p. (b) miRNAs down-regulated in Oct4+ hDFs: miR-34c-5p, miR-193a-5p, miR-424-5p, miR-503-5p, miR-143-3p and miR-145-5p. The results are shown as expression relative to corresponding control GFP+ hDFs. Error bars represent ± SD.