Fig. 2.

Assigning the chemical shifts of GGM in spruce. a The carbon 1-2 region of ¹³C CP-INADEQUATE MAS NMR spectra of wild type Arabidopsis and the csla2/3/9 mutant. The GGM carbons 1 and 2 are labelled. The wild type spectrum was previously published in (Grantham et al., 2017)²³. b The acetate methyl (Ac^Me) and carbohydrate regions of a 30 ms mixing time ¹³C CP-PDSD MAS NMR spectra of spruce is shown. The intramolecular cross-peaks of two GGM mannose backbone residues are labelled. For GGM, the acetylated mannosyl residue carbons are labelled Ac; where they share the same chemical shift as the unacetylated mannose carbons they are labelled [Ac]. Spinning side bands are marked SSB