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. 2019 Oct 31;9:15725. doi: 10.1038/s41598-019-51749-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glucosinolate sulfatase (GSS) activity in Psylliodes chrysocephala adults. (a) Crude tissue homogenates were prepared from dissected guts and the remaining body tissues, and gut tissue homogenates were fractionated into membrane fraction and soluble protein fraction by centrifugation. Samples were incubated with a mixture of eight different glucosinolates (GLS) for 2 h at 35 °C, and desulfo-glucosinolates were quantified by LC-MS/MS using external standard curves. (b) Comparison of GSS activity in crude gut homogenates towards eight different glucosinolates. Means + SD of n = 4 biological replicates. n.d., not detected; 2OH3But, 2-hydroxy-3-butenyl; 3But, 3-butenyl; 4MSOB, 4-methylsulfinylbutyl; 4MTB, 4-methylthiobutyl; I3M, indol-3-ylmethyl; 2PE, 2-phenylethyl.