Table 2.
Glucosinolate sulfatase activity of recombinant enzymes towards different glucosinolates (GLS).
| GLS | pmol desulfo-GLS in 2 h (mean ± SD; n = 3) | ||||
|---|---|---|---|---|---|
| PcGSS1 | PcGSS2 | PcGSS3 | PcGSS4 | PcGSS5 | |
| 2OH3But | n.d. | n.d. | n.d. | n.d. | n.d. |
| 3But | n.d. | n.d. | 4.18 ± 1.95 | 20.39 ± 3.88 | n.d. |
| 4MSOB | n.d. | 3.04 ± 0.32 | n.d. | n.d. | n.d. |
| 4MTB | n.d. | n.d. | 3.09 ± 0.43 | 0.48 ± 0.32 | n.d. |
| I3M | n.d. | 13,804.15 ± 582.38 | n.d. | n.d. | n.d. |
| 2PE | n.d. | 1.73 ± 0.08 | 3.62 ± 2.42 | 6.55 ± 4.25 | tr. |
| Benzyl | 1.74 ± 0.94 | 13.47 ± 0.65 | n.d. | n.d. | n.d. |
| Sinalbin | 91.41 ± 40.18 | 6.60 ± 0.51 | n.d. | n.d. | n.d. |
2OH3But, 2-hydroxy-3-butenyl; 3But, 3-butenyl; 4MSOB, 4-methylsulfinylbutyl; 4MTB, 4-methylthiobutyl; I3M, indol-3-ylmethyl; 2PE, 2-phenylethyl; n.d., not detected; tr., trace amount.