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. 2019 Oct 31;9:15765. doi: 10.1038/s41598-019-52323-1

Figure 6.

Figure 6

AGT and SGLT2 expression in HK-2 with or without HNRNPF KO. (A) WB, (B–D) semi-quantitation of WB and (E–G) RT-qPCR of HNRNPF, AGT, SGLT2 and β-ACTIN in different clones of HK-2 Ctrl and HK-2 with HNRNPF KO by CRISPR gRNA. Values are means ± SEM, n = 3. *P < 0.05, **P < 0.01; HK-2-HNRNPF KO versus HK-2 Ctrl. (H) WB, (I–K) semi-quantitation of WB and (L)(M)(N) RT-qPCR of HNRNPF, AGT, SGLT2 and β-ACTIN of expression in HK-2 with or without canagliflozin (Cana) (0.5 mM) treatment for 24 hours. Values are means ± SEM, n = 3. NS, not significant. HK-2-Cana versus HK-2 Ctrl.