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. 2019 Oct 31;9:15771. doi: 10.1038/s41598-019-52364-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Domain mapping analysis of the VEGFA–calpain-6 interaction using the yeast two-hybrid assay. (a) The left panel is a schematic diagram showing the respective cDNA constructs for the VEGFA full-length and deletion mutants constructed by standard cloning and then verified by DNA sequencing. In the right panel, yeast transformants were assayed for their ability to grow on medium lacking leucine (upper panel) and for galactosidase expression through the formation of a blue colony on the plate containing X-gal (bottom panel). The values of β-galactosidase activity (unit), examined by adding o-nitrophenyl β-D-galactopyranoside (ONPG) agents, are indicated below their corresponding lanes. The data represent three independent experiments and are shown as means ± SD. (b) Identification of the interacting domains between calpain-6 and VEGFA through a yeast two-hybrid analysis. The left panel displays a schematic representation of the cDNA constructs for the full-length and truncated mutant calpain-6 fusion proteins. The right panel shows the results of the protein–protein interactions assessed in the yeast two-hybrid assay. The yeast transformants were assayed for their ability to grow on medium lacking leucine (upper panel) and for galactosidase expression through the formation of a blue colony on a plate containing X-gal (bottom panel). (c) Biological interactions between the cDNA constructs for VEGFA (Asn101–Arg147) and three calpain-6 deletion proteins (Met¹–Asp³²⁰, Gly³²¹–Asp⁵⁰⁰, Met⁵⁰¹–Leu⁶⁴¹), as determined by the yeast two-hybrid analysis. The C-terminal of VEGFA interacted with the domain comprising the amino acid region 321–500 in calpain-6.