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. 2019 Oct 31;10:4970. doi: 10.1038/s41467-019-12956-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — NDH-2 is required for redox homeostasis and counteracts inhibition of NDH-1. a Impact of rotenone on growth. b Survival in fatty-acid-free Sauton’s minimal medium supplemented with 50 μg/mL rotenone. c Impact of oleic acid on growth. (d) NADH/NAD + intracellular ratio after 6 h in modified Sauton’s minimal medium supplemented with DMSO (1%, control), or oleic acid (500 μM). Impact of valinomycin (e), nigericin (f), and rifampicin (g) on growth in modified Sauton’s minimal medium supplemented with DMSO (1%; control), or oleic acid at different concentrations (100 μM, 150 μM, and 200 μM). h Proposed mechanism of NDH-2 essentiality in the presence of fatty acids. Data are averages of three data points and representative of at least two independent experiments. Error bars correspond to standard deviation. “Comp” stands for complemented. Statistical significance was assessed by one-way ANOVA followed by post hoc test (Tukey test; GraphPad Prism). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Source data are provided as a Source Data file