Fig. 3.

Representative images of TUNEL staining in ovarian tissues on PND70. Increased cell death was observed in ovarian follicles after galactose exposure. Strikingly, hardly any TUNEL-positive cells were detected after DHEA treatment. The red signal indicates TUNEL-positive staining, and the blue signal (DAPI) indicates nuclei. (N = 6 per groups). Graph shows quantification of average percentage of TUNEL-positive cells in ovaries per experimental group. # indicates significant difference from control; † indicates significant difference from gal-exposed; ‡ indicates significant difference from the vehicle. ANOVA, P < 0.05. Scale bars = 50 μm