Table 3.

Quantification of the amount of enzyme washed-off during rinsing.

Sample	Total enzyme washed away during rinse (mU/5 cm2)a	% of enzyme washed away during rinseb	Actual loading of enzymes (mU/5 cm2)c
PN@AP/GOx	211	35	385
PN@CRP/GOx	168	25	485
PN@AP-PEG/GOx	150	29	368
PN@AP-PAM/GOx	176	27	465
PN@CRP-PEG/GOx	157	28	394
PN@CRP-PAM/GOx	191	23	565

Note: ^aTotal washed-off active enzyme was the addition of all active enzymes washed during each rinse.

^bPercentage of active enzyme washed away was calculated against the immobilization of active enzyme.

^cThe actual loading of enzymes were calculated by subtracting the total amount of enzyme washed away during rinse from initial loading.