Table 1.
Oligonucleotides used for CDV P gene detection and for full length H gene and Fsp-coding region amplification and sequencing.
| Oligonucleotide label | Oligonucleotide sequence | Genomic position* | Reference |
|---|---|---|---|
| Amplification of P gene | |||
| CDV Universal (forward) | ATGTTTATGATCACAGCGGT | 2132–2151 | Daly et al., 2006 |
| CDV Universal (reverse) | ATTGGGTTGCACCACTTGTC | 2541–2560 | Daly et al., 2006 |
| Amplification and sequencing of H gene | |||
| CDVff1 (forward) | TCGAAATCCTATGTGAGATCACT | 6897–6919 | Lan et al.32 |
| CDVHS2 (reverse) | ATGCTGGAGATGGTTTAATTCAATCG | 8994–8969 | Lan et al.32 |
| CDVHS1 (forward) | AACTTAGGGCTCAGGTAGTCC | 7054–7074 | Lan et al.32 |
| CDVHforD (forward) | GACACTGGCTTCCTTGTGTGTAG | 7948–7970 | Lan et al.32 |
| CDVHr2 (reverse) | GTTCTTCTTGTTTCTCAGAGG | 8198–8178 | Lan et al.32 |
| CDVP2F (forward) | ACTTCCGCGATCTCCACT | 7372–7389 | Pardo et al.33 |
| CDVP3R (reverse) | ACACTCCGTCTGAGATAGC | 7760–7742 | Pardo et al.33 |
| CDVP5R (reverse) | GTGAACTGGTCTCCTCTA | 8395–8378 | Pardo et al.33 |
| Amplification and sequencing of Fsp-coding region | |||
| F5 (forward) | TGTTACCCGCTCATGGAGAT | 4272–4292 | Riley and Wilkes, 2015 |
| R5 (reverse) | CCAAGTACTGGTGACTGGGTCT | 5411–5433 | Riley and Wilkes, 2015 |
| CDV-F4854 (Forward) | TCCAGGACATAGCAAGCCAACA | 4854–4875 | Sarute et al., 2013 |
| CDV-R5535 (Reverse) | GGTTGATTGGTTCGAGGACTGAA | 5513–5535 | Sarute et al., 2013 |
*Reference genome AF164967 (A75/17).