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. 2019 Oct 31;9:15789. doi: 10.1038/s41598-019-52422-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Taxonomic classification of eukaryotic reads in protozoa-rich bovine faecal samples (MB1) at SILVA levels 4 and 7. Circles from the inside show taxonomic distributions using the EMP (1391F/EukBr), 563F/1132R, 616*F/1132R, 1183F/1631R, DM568F/RM2F, RM2F/RM3R, GA12F/RM4R and GA20F/RM9R primer sets, respectively. Only taxa having ≥5% of the total non-bacterial reads are shown in the plots, and taxa with <5% are summarised in ‘Others’. Sequence reads without taxonomic assignments because of low similarity with the database are shown as ‘Unassigned’.