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. 2019 Oct 31;9:15761. doi: 10.1038/s41598-019-52339-7

Figure 5.

Figure 5

Oleate, but not palmitoleate, deactivates mTORC1 and resolves ER stress in vivo. (A) 10–12 weeks old male WT, SCD1 GKO, GLS5, and GLS3 were fed HCD for 10 days. Western blot with the indicated antibodies performed on liver samples. (B,C) 12 weeks old male LOX and LKO mice were fed HCD, H-18:1, or H-18:0 for 10 days. Western blot analysis with the indicated antibodies and liver gene expression analysis of ER stress genes using Qpcr. Values are mean ± SEM (n = 3–6/group), *P < 0.05 vs LOX counterparts and #P < 0.05 vs vehicle treated LKO by student’s two-tailed t test. (D) Proposed mechanism: Hepatic SCD1 deficiency increases palmitate which subsequently promotes mTORC1-mediated ER stress through PGC-1α.