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. 2019 Oct 31;8(1):169–184. doi: 10.1089/biores.2018.0048

FIG. 3.

FIG. 3.

(a): Cultures of T-47D cells with BPA were performed as described in Figures 1a and 2a. Cells were then treated with 600 nM BPA for the various time intervals indicated. Cellular extracts were prepared and subjected to protein quantification, SDS-PAGE, and Western blot analyses. The relative intensity of p53, compared to Cs, is displayed as the mean ± SEM. The sample sizes came from five density measurements per group. The asterisk indicates significant difference with the control at p < 0.05 (Kruskal–Wallis test followed by post hoc analysis using Mann–Whitney U-test). Representative Western blots are shown. (b) Cultures of T-47D cells with BPA were performed as described in Figures 1a and 2a. Cells were then treated with 600 nM BPA for the various time intervals indicated. Cellular extracts were prepared and subjected to protein quantification, SDS-PAGE, and Western blot analyses. The relative intensity of ERα, compared to Cs, is displayed as the mean ± SEM. The sample sizes came from five density measurements per group. The asterisk indicates significant difference with the control at p < 0.05 (Kruskal–Wallis test followed by post hoc analysis using Mann–Whitney U-test). Representative Western blots are shown.