FIG. 4.

(a) Culture and treatment of T-47D cells were performed as described in Figures 1a and 2a. Cells were treated for 24 h with BPA (600 nM), E₂ (1 nM), ICI (1 μM), TAM (1 μM), and RAL (1 μM). Cellular extracts were prepared and subjected to protein quantification, SDS-PAGE, and Western blot analysis. Actin bands are shown to indicate equal protein loading among treatment conditions. The relative intensity of p53, compared to Cs, is displayed as the mean ± SEM. The sample sizes came from five density measurements per group. The asterisk indicates significant difference with the control at p < 0.05 (Kruskal–Wallis test followed by post hoc analysis using Mann–Whitney U-test). Representative Western blots are shown. (b) Culture and treatment of MCF-7 cells were performed as described in Figures 1b and 2b. Cells were treated for 24 h with BPA (600 nM), E₂ (1 nM), ICI (1 μM), TAM (1 μM), and RAL (1 μM). Cellular extracts were prepared and subjected to protein quantification, SDS-PAGE, and Western blot analysis. Actin bands are shown to indicate equal protein loading among treatment conditions. The relative intensity of p53, compared to Cs, is displayed as the mean ± SEM. The sample sizes came from five density measurements per group. The asterisk indicates significant difference with the control at p < 0.05 (Kruskal–Wallis test followed by post hoc analysis using the Mann–Whitney U-test). Representative Western blots are shown. E₂, 17β-estradiol; RAL, raloxifene; TAM, tamoxifen.