FIG. 7.

MCF-7 cells were cultured in 12-well plates containing 30,000 cells per well. Cells were grown for 2 days in 10% FBS media containing growth factors. For the following 6 days, growth factor media was replenished with DCC-FBS media and treated with ligands at 2-day intervals over 6 days. The treatments consisted of various combinations of BPA (600 nM), E₂ (1 nM), ICI (1 μM), TAM (1 μM), and RAL (1 μM). A cellular viability assay was performed on the seventh day utilizing propidium iodide staining and image cytometry using the Nexcelom Cellometer Vision. Single asterisks are indicative of significant difference with comparison to the control at p < 0.05 and ***p < 0.001 (Kruskal–Wallis test followed by post hoc analysis using Mann–Whitney U test). Three independent experiments are displayed in the graph.