FIG. 8.

Treated T-47D cells were grown in 12-well growth plates, each well contained ∼30,000 cells on cover-slips. The cells were nourished for 2 days in whole media containing 10% FBS. They were then withdrawn from endogenous growth factors by culturing in DCC-FBS for 6 days. E₂, BPA, ICI, RAL, and TAM were added in 2-day intervals for a period of 6 days. Cells were treated with Cy3 (red) and DAPI (blue) immunofluorescent stains, and the cytolocalization of p53 was determined using confocal microscopy. From the confocal microscopic images it is determined that p53 is located within the nuclei of T47D cells in all of the conditions. DAPI, 4′,6-diamidino-2-phenylindole.