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. 2019 Oct 25;10:2406. doi: 10.3389/fmicb.2019.02406

FIGURE 1.

FIGURE 1

Infection with Clostridium perfringens induces inflammasome activation with caspase-1 activation, IL-1β release and pyroptosis in macrophages. BMDMs from C57BL/6 mice were primed with LPS (100 ng/ml) for 2 h and infected with C. perfringens (strain 13 or ATCC13124) for the indicated times, or treated with 10 μM nigericin for 30 min as a positive control. (A) Phase- contrast images of uninfected (-) or strain 13-infected BMDMs at 60 mpi. Bar, 100 μm. (B) Culture supernatants of the infected BMDMs were analyzed for LDH release at 20–60 mpi. (C) Activation of caspase-1, and cleavage of IL-1β or GSDMD in the infected BMDMs was analyzed by immunoblotting with anti-caspase-1 (p20), anti-IL-1β and anti-GSDMD antibody, respectively. The levels of β-actin as the loading control were also examined. (D) IL-1β release from the infected BMDMs into the culture supernatants at 20–60 mpi was analyzed by an ELISA. Data are presented as the means ± SD of triplicate samples.