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. 2019 Oct 25;10:2406. doi: 10.3389/fmicb.2019.02406

FIGURE 3.

FIGURE 3

Clostridium perfringens triggers inflammasome activation through the NLRP3-ASC axis. BMDMs from wild-type, NLRP3- (Nlrp3–/–)- or ASC- (Pycard–/–) deficient mice were primed with LPS for 2 h and infected with WT C. perfringens (strain 13) for 1 h, or treated with 10 μM nigericin for 30 min as positive controls (A,D,E). The BMDMs were infected with C. perfringens, an SPI-2-deficient mutant of Salmonella for 1 h or treated with nigericin in the presence or absence of KCl (130 mM) (D,E). (A,D) The activation of caspase-1, and cleavage of IL-1β or GSDMD in the infected BMDMs were analyzed by immunoblotting with anti-caspase-1 (p20), anti-IL-1β or anti-GSDMD antibody, respectively. (B,E) IL-1β release from the infected BMDMs into the culture supernatants at 1 hpi was analyzed by an ELISA. Data are presented as the means ± SD of triplicate samples. (C) Culture supernatants of the infected BMDMs were analyzed for LDH release at 1 hpi.