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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Mol Microbiol. 2019 Aug 27;112(4):1326–1338. doi: 10.1111/mmi.14365

Figure 3.

Figure 3.

The eptA homolog of V. fischeri (VF_A0210) is differentially expressed under acidic conditions and acts as a lipid A ethanolamine transferase. (A) pH-dependency of PMB MIC assay performed on wild-type V. fischeri ES114 (WT) and its derivatives, with or without the eptA gene. Bars represent mean with 95% confidence intervals (CI). (B) Transcription of eptA over time after exposure to acidic or basic pH. Points represent mean + SD. Data represent at least three biological replicates, *: p≤0.1 according to Mann-Whitney test. (C) Representative mass spectrometry spectra of lipid A extracts isolated from either wild-type V. fischeri or its ΔeptA derivative, grown at pH 6.5 or 8.0. Arrows indicate 43 m/z shifts, consistent with a dehydration addition of an ethanolamine residue.