Fig. 5.

Upon okadaic acid (OA) (PP2A inhibitor) pretreatment, apoptosis and cell damage were reversed to an extent in L02 cells exposed to serum deprivation and hypoxia, so was Akt inactivation; while all the phenotypes became more severe upon D-erythro-S (DES) (PP2A activator) pretreatment. OA was used at a concentration of 10 nM and DES at a concentration of 5 μM. The cells were pretreated with the compounds for 60 min before exposure to serum deprivation and hypoxia. a Representative FACS plots of apoptosis detection were shown. b From left to right: Quantitative analysis of the apoptotic rate; LDH measurement, the unit: U/L; The PP2A activity and Akt activity measurement normalized to the untreated 0 h group. All data were expressed as the mean ± SEM. n = 3 for each group in each measurement. *p < 0.05, and ***p < 0.001 versus untreated cells at the same time; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, and ^####p < 0.0001, 12 h versus 0 h, with the same treatment