Figure 7.

Effect of Hydrogen Peroxide on Viability and Protein Synthesis in S. pombe cmk2 Mutants

(A) Cells were treated with increasing concentrations (0–1 mM) of H₂O₂ for 1 h. An equal number of cells were plated and grown for 3 days, and colonies were counted. The graph represents the mean ± standard deviations of at least two independent experiments each done in triplicates.

(B) Cells were treated with 1 mM H₂O₂ or 50 μg/mL CHX or untreated. After 15 min, [³⁵S]-methionine was added for an additional 45 min. Protein synthesis levels were quantified and adjusted according to the total amount of protein. The graph represents the normalized mean ± standard deviations of two independent experiments each done in triplicates. The individual symbols represent summaries of the individual experiments. *p value eEF2K^−/− = 1.2 × 10⁻⁹, p value eIF2α^S51A/eEF2K^−/− = 9.4 × 10⁻⁹. Statistical significance was determined by t test.

(C) Cells were treated with 1 mM H₂O₂ for the periods indicated and labeled with [³⁵S]-methionine for 5 min. Protein synthesis was quantified and normalized to the total amount of protein. The graph represents the normalized mean ± standard deviations of at least two independent experiments each done in triplicates.