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. 2019 Apr 20;43(4):645–653. doi: 10.1016/j.jgr.2019.04.005

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© 2019 The Korean Society of Ginseng, Published by Elsevier Korea LLC.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 1 — Ginseng-derived cytochrome P450 PgCYP736A12 is closely related to other CYP family proteins. (A) Phylogenetic tree of PgCYP736A12 protein with other closely related cytochrome P450 proteins in plants. The phylogenetic tree was constructed by the neighbor-joining method using the ClustalX program. At, Arabidopsis thaliana; Ht, Helianthus tuberosus; Sm, Swertia mussotii; Cr, Catharanthus roseus; Ph, Petunia hybrid; Sm, Solanum melongena; Gm, Glycine max; Nt, Nicotiana tabacum; Ta, Triticum aestivum; Pg, Panax ginseng; Vv, Vitis vinifera; Bc, Bupleurum chinense; Sa, Sorbus aucuparia, Md, Malus domestica. The gene accession numbers are as follows: AtCYP76C2 (AT2G45570), AtCYP76C4 (AT2G45550), AtCYP76C1 (AT2G45560), AtCYP76C7 (AT3G61040), HtCYP76B1 (Y10098), SmCYP76B10 (GU168041), CrCYP76B6 (AJ251269), PhCYP76A4 (AB016061), SmCYP76A1 (X71658), GmF3′,5′H (GLYMA_13G072100), HtCYP81B1 (AJ000478), AtCYP81F2 (NM_125104), NtCYP71AH11 (GU590868), GmCYP71A10 (GLYMA_06G176100), TaCYP71C6v1 (BAF97103), BcCYP736A53 (JF803814), BcCYP736A54 (AFK79031), VvCYP736B (ACM89789), SaCYP736A107 (AHN09742), MdCYP736A163 (AKB94045), HtCYP73A1 (Z17369), AtCYP83A1 (NM_117451), AtCYP83B1 (NM_119299), VvCYP716B2 (VIT_00035577001). The bar represents 0.1 substitution per amino acid position. PgCYP736A12 New are marked with red square box. Blue square–boxed proteins are reported to be involved in herbicide tolerance. (B) Sequence alignment of PgCYP736A12 with other close homologs. Black boxes indicate identical residues, and similar residues are shaded in gray. The four red-box motifs represent the oxygen-binding domain (A/G)GX(D/E)T(T/S), K-helix region (EXXR), domain C (PER), and heme-binding region (FXXGXRXCXG). Gaps were inserted to maximize homology.

Fig. 1 — Ginseng-derived cytochrome P450 PgCYP736A12 is closely related to other CYP family proteins. (A) Phylogenetic tree of PgCYP736A12 protein with other closely related cytochrome P450 proteins in plants. The phylogenetic tree was constructed by the neighbor-joining method using the ClustalX program. At, Arabidopsis thaliana; Ht, Helianthus tuberosus; Sm, Swertia mussotii; Cr, Catharanthus roseus; Ph, Petunia hybrid; Sm, Solanum melongena; Gm, Glycine max; Nt, Nicotiana tabacum; Ta, Triticum aestivum; Pg, Panax ginseng; Vv, Vitis vinifera; Bc, Bupleurum chinense; Sa, Sorbus aucuparia, Md, Malus domestica. The gene accession numbers are as follows: AtCYP76C2 (AT2G45570), AtCYP76C4 (AT2G45550), AtCYP76C1 (AT2G45560), AtCYP76C7 (AT3G61040), HtCYP76B1 (Y10098), SmCYP76B10 (GU168041), CrCYP76B6 (AJ251269), PhCYP76A4 (AB016061), SmCYP76A1 (X71658), GmF3′,5′H (GLYMA_13G072100), HtCYP81B1 (AJ000478), AtCYP81F2 (NM_125104), NtCYP71AH11 (GU590868), GmCYP71A10 (GLYMA_06G176100), TaCYP71C6v1 (BAF97103), BcCYP736A53 (JF803814), BcCYP736A54 (AFK79031), VvCYP736B (ACM89789), SaCYP736A107 (AHN09742), MdCYP736A163 (AKB94045), HtCYP73A1 (Z17369), AtCYP83A1 (NM_117451), AtCYP83B1 (NM_119299), VvCYP716B2 (VIT_00035577001). The bar represents 0.1 substitution per amino acid position. PgCYP736A12 New are marked with red square box. Blue square–boxed proteins are reported to be involved in herbicide tolerance. (B) Sequence alignment of PgCYP736A12 with other close homologs. Black boxes indicate identical residues, and similar residues are shaded in gray. The four red-box motifs represent the oxygen-binding domain (A/G)GX(D/E)T(T/S), K-helix region (EXXR), domain C (PER), and heme-binding region (FXXGXRXCXG). Gaps were inserted to maximize homology.