Fig. 3.

The effects of CK on AKT activation and metastasis. (A) SKBR3 cells were treated with shAKT1 or shAKT2 in the presence or absence of CK (25 μM) for 24 h, and cell proliferation was determined by MTT assay. (B) The invasion capacity of SKBR3 cells treated with various dosages of CK (0–25 μM) for 24 h was analyzed by hematoxylin and eosin staining. Quantification of invasion capacity was performed using Image J software. (C) The effects of CK on cell migration were evaluated by wound healing assay. A wound was created by scraping the cell monolayer using a pipette tip. Photographs were captured using a digital camera after 24 h of treatment with CK (0–25 μM). (D) In the clonogenic assay, SKBR3 cells were seeded on plates and incubated until the colonies formed. The colonies were stained with 0.5% crystal violet, and their areas were measured using Image J software. *p < 0.05, **p < 0.01 compared with control. CK, Compound K; MTT, 3-(4-5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide.