Fig. 6.

The N169 glycan is a complex type. A, Ribbon diagram of CD16a highlighting N169. B, The two different CD16a isolation techniques generate two different glycopeptides that show similar processing. The predominant N169 glycans from CD16a released with PMA ionizes well. These forms account for 96.2% of the total observed N169 intensity from the NK12 and NK13 donors (N169 glycopeptide 2). C, N169 glycopeptides isolated from CD16a purified using detergent lysis of NK cells provided fewer observations from the NK08 and NK11 donors (N169 glycopeptide 1). D, CD16a glycopeptides from the NK09 donor showed a peptide lacking the L residue. N-glycan cartoons represent one possible configuration based on composition. See also Table II and supplemental Fig. S4.