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. 2019 Jul 15;18(11):2310–2323. doi: 10.1074/mcp.TIR119.001433

Fig. 4.

Fig. 4.

The interleukin receptor IL20RA is a receptor for the immune checkpoint B7-H3. A, B7-H3 was expressed as a recombinant protein and screened using the Conditioned Media AlphaScreen technology. The B7-H3 STM receptor interactome identifies IL20RA as a top hit. Proteins labeled in gray color represent nonspecific binders. S/N ratio ≥ 20 was used as experimentally-determined cut-off for hit calling (dotted gray line). B, B7-H3 binding to IL20RA on the cell surface. Binding of recombinant biotinylated B7-H3 to cells expressing IL20RA or control receptors (IL20RA, IL20RB, IL17RB, IL10RA, IL10RB, LIFR, OSMR, and IL6R) was analyzed by immunofluorescence, using fluorophore-conjugated streptavidin for detection. Intersection plot shows B7-H3 binding to the surface of cells expressing the indicated receptors; transfections were performed in duplicates. Micrographs show images corresponding to B7-H3 binding to IL20RA- and IL20RB-expressing cells, as control. Scale bar = 50 μm.