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. 2019 Jul 15;18(11):2310–2323. doi: 10.1074/mcp.TIR119.001433

Fig. 5.

Fig. 5.

The orphan NK cell receptor KIR2DL5A is a novel binding partner for PVR. A, PVR screens using the Conditioned Media AlphaScreens platform identify known interacting partners as well as a previously unknown interaction with the receptor KIR2DL5A (inset). B, Analysis of PVR binding to KIR2DL5A, expressed as recombinant purified proteins, by BLI. Biotinylated PVR was immobilized on streptavidin sensors and tested for binding to KIR2DL5, as a soluble ECD-Fc tag analyte, at the indicated concentrations. C, PVR binding to receptors expressed on the cell surface. Individual KIR receptors (KIR2DL1, KIR2DL2, KIR2DL3, KIR2DL4, KIR2DL5A, KIR2DL5B, KIR2DS1, KIR2DS3, KIR2DS4, KIR2DS5, KIR3DL1, KIR3DL3) or known PVR binders (CD96, CD226, TIGIT, PVRL3, and PVRL4) were transiently expressed in cells, and binding of PVR to the cell surface was analyzed by immunofluorescence using fluorescent streptavidin for detection. Binding is represented as signal intensity/noise, and shown as intersection plots representing two independent transfections. Representative images for PVR binding to the cell surface are shown.