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. 2019 Oct 25;9:364. doi: 10.3389/fcimb.2019.00364

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Copyright © 2019 Metz, Tjan, Wu, Pervaiz, Hermans, Shettigar, Sears, Ritschel, Dutilh and Boleij.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CDCA interacts directly with recombinant BFT-1. TSA with CDCA and rBFT-1 (N = 3), where CDCA resulted in a significant shift in melting temperature at concentrations of 85 and 175 μM (ΔTm 1.10 and ΔTm 2.10, respectively (P < 0.0001 for 1-Way ANOVA and Dunnett's multiple comparison test) when compared to the negative control condition (CDCA 0 μM). CDCA at a concentration of 8.5 μM did not result in a significant shift of the melting temperature of rBFT-1. Bars indicate means and standard deviations. N = 4–12 for each condition.