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. 2019 Oct 25;10:2425. doi: 10.3389/fmicb.2019.02425

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Copyright © 2019 Jing, Li, Zhang, Wang, Wu, Li, Xie and Qing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypes caused by transient expression of the MaYVV C4 protein. (A) Phenotypes of N. benthamiana plants infected with PVX alone or with PVX/C4. The plants infected with PVX/C4 showed leaf upward curling and abnormal flowering symptoms. Photographs were taken at 30 dpi. (B) RT-qPCR analyses of PVX accumulation in the systemic leaves of the PVX- or PVX/C4-infected N. benthamiana at 15 dpi. Expression of Nbactin was used as an internal control. ^∗indicates the two treatments differ significantly (P value < 0.05 by the Student’s t-test). The experiment was repeated three times with similar results.