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. 2019 Oct 25;10:2425. doi: 10.3389/fmicb.2019.02425

FIGURE 2.

FIGURE 2

Disease symptoms and viral/betasatellite DNA accumulations in the MaYVV-, MaYVVΔC4-, MaYVV/MaYVB-, and MaYVVΔC4/MaYVB-infected plants. (A) A schematic representation of mutations introduced into the C4 gene. The two potential translation start codons (ATG) in the C4 gene were mutated into ACG without changing the amino acid sequence of the C1 protein. (B) Symptoms induced by inoculations of N. benthamiana plants with MaYVV, MaYVVΔC4, MaYVV/MaYVB and MaYVVΔC4/MaYVB at 15 dpi. No obvious symptoms were observed in either MaYVV- or MaYVVΔC4-infected plants. Symptoms in the MaYVVΔC4/MaYVB co-infected plants were delayed and alleviated compared with that shown by the MaYVV/MaYVB co-infected plants. (C,D) Quantitative PCR analyses of MaYVV and MaYVB DNA copy numbers in the systemically infected leaves at 15 dpi. ∗indicates significantly different between the treatments (P value < 0.05 by the Student’s t-test); ∗∗indicates extremely significant differences between the treatments (P < 0.01 by the Student’s t-test) by the Student’s t-test. (E) Southern blot analyses of MaYVV and MaYVB accumulations in the systemically infected leaves at 15 dpi. The blot was probed with specific probes of MaYVV and MaYVB. Genomic DNA was visualized by GoldView staining and was used to show sample loadings.