Skip to main content
. 2019 Oct 25;10:2425. doi: 10.3389/fmicb.2019.02425

FIGURE 5.

FIGURE 5

MaYVV C4 protein can suppress PTGS. (A) 16c N. benthamiana leaves were agro-infiltrated with pCHF3 and pCHF3/eGFP (pCHF3 + 35S: GFP), pCHF3/C4 and pCHF3/eGFP (pCHF3/C4 + 35S: GFP) or pCHF3/P19 and pCHF3/eGFP (pCHF3/P19 + 35S: GFP). The infiltrated leaves were photographed at 3 dpi. One infiltrated leaf of each plant was detached and photographed again (lower panel). (B) Northern blot analysis of GFP mRNA accumulation in different agro-infiltrated leaves. GoldView-stained rRNA is used to show sample loadings. (C) Western blot analysis of GFP protein accumulation in different agro-infiltrated leaves using a GFP specific antibody followed by a HRP-conjugated goat-anti-rabbit antibody. The Coomassie brilliant blue-stained rubisco was used to show sample loadings.