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. 2019 Oct 14;43(5):293–304. doi: 10.3906/biy-1905-6

Figure 3.

Figure 3

E2F6 expression is increased in breast cancer compared to normal breast tissue: (A) 48 breast tissue cDNAs were used to investigate the fold change in cDNA levels of E2F6 using three sets of primers: E2F6# com2 (common to all E2F6 variants), E2F6# var a (specific for variant a), and E2F6# var b (specific for variant b). Results were first normalized to β-actin and then shown relative to the median E2F6 expression level across 5 normal breast cDNAs. The same data are displayed according to tumor stage (B). The same data were stratified according to ER status (C). Each point represents the average of two technical repeats. Dots are individual samples; the lines refer to the median value. P-values (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001) were measured by Mann–Whitney U test.