Fig. 4.

Increased efficacy with combined treatment of 5-Aza-2′-deoxycytidine and curcumin on alternative splicing. (a-b) RPS16 normalized qRT-PCR HNC cells treated with Aza + curcumin combination for 48 h using the indicated primers. (c) MeDIP in HNC cells treated with Aza + curcumin combination for 48 h and qRT-PCR relative to input and control IgG. Three independent experiments were conducted with mean values ± SD. P-value calculated using two-tailed Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001, ns = non significant