Fig. 6.

Effect of curcumin on global alternative splicing events. (a-c) Schematic representation of (a)TBC1D4, (b) VPS39, (c) ZNF207 and their cancer-specific spliced isoform. RPS16 normalized qRT-PCR in curcumin-PEC treated and control-PEC in H157 cells using the indicated exon junction specific primers for the respective genes. MeDIP in curcumin-PEC treated and control-PEC in H157 cells and qRT-PCR in respective genes relative to input. ChIP in curcumin-PEC treated and PEC-control H157 cells using RNA Pol II and BORIS antibody, followed by qRT-PCR relative to input. Three independent experiments were conducted with mean values ± SD. P-value calculated using two-tailed Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001, ns = non-significant