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. 2019 Oct 31;9(4):2045894019883613. doi: 10.1177/2045894019883613

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 1. — Complete flushing of blood from the lungs is required for optimal contrast perfusion. (a) Gravity-assisted formalin instillation device set to fill lungs post-contrast perfusion at 15 cmH₂O. (b) After perfusing the lungs with barium, 10% phosphate buffered formalin is instilled into the lungs via the intubation tube after disconnecting from the ventilator. Well-flushed, barium-gelatin-perfused lungs are removed en bloc after formalin instillation (c, d). (c) Lungs from normal controls appear brilliant white following the perfusion process. (d) Lungs from PAH animals appear pinker after the perfusion process. (e) Lungs are stored in formalin at 4℃ for two days before PBS rinse and long-term storage in 70% ethanol.