Fig. 3.

MiR-206 suppresses PTP1B expression via directly targeting PTP1B mRNA 3′UTR. a A model demonstrates the predicted conserved miR-206 binding site in the 3′UTR of PTP1B mRNA. Schematic diagram shows the miR-206 binding site-directed mutant of PTP1B mRNA 3′UTR. The PTP1B mRNA 3′UTR fragment containing wild type or mutant of miR-206-binding site was cloned into the downstream of the luciferase reporter gene in pGL3-control vector. b, c In MCF-7 cells regulation of miR-206 (or anti-miR-206) on pGL-PTP1B-wt and pGL-PTP1B-mut was tested by using luciferase reporter gene assay. d, e Effect of miR-206 (or anti-miR-206) on PTP1B expression was examined by Western blot. The level of miR-206 was analyzed by qRT-PCR assay after the cells were transfected with miR-206 or anti-miR-206. Statistical significant differences are indicated: **p < 0.01 and no significance (NS), Student’s t-test